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Designing and interpreting large-scale experiments to understand pathway
structure and its relationship to phenotype and human disease.
Current experimental interests:
- Developing technologies to efficiently phenotype many gene deletion combinations in S. cerevisiae under multiple growth conditions, with initial application
to genes involved in transcription and DNA repair.
- En masse protein interaction screening technologies using next-generation sequencing of recombinant DNA barcodes.
- Systematic development of yeast complementation assays to assess human functional variation.
Current computational interests:
- Systematic analysis of genetic interaction to reveal redundant systems and order of action in genetic pathways.
- Integrating large-scale studies — including phenotype,
genetic epistasis, protein-protein and transcription-regulatory
interactions and sequence patterns — to quantitatively assign
function to genes and guide experimentation and disease
association studies.
- Analysis of allele-dependent, environmental or co-factor-dependent changes in protein and genetic interaction networks
Information on positions available in the Roth lab.
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